It is not clear and little difficult to know that when a formulation administered, active principles in the formulation gain entry in to the systemic circulation or not. The present study was planned to acquire some preliminary data with regards to the absorption of bacoside from the Brahmi Ghrita formulation when administered orally in acute and chronic period. Brahmi Ghrita was administered for single dose i.e 1 day and also chronic dose for seven days to rats. Blood samples were withdrawn after 1 hr and 3 hr intervals of drug administration and presence of bacoside was detected in serum sample using High Performance Thin Liquid Chromatography.\r\nBacoside present in Brahmi Ghrita was found to get absorbed in to systemic circulation in both the groups. Maximum concentration in serum was seen in the animal after 1 hr of drug administration in comparison to animal after 3 hr of drug administration in both the acute and chronic phase. From the study it can be said that, phytochemical of the Brahmi Ghrita formulation do get absorbed in systemic circulation after 1 hr of drug administration. The simple, accurate, cost effective HPTLC method can be utilized to detect bacoside in serum.
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